Journal: Pharmaceutics

Article Title: Synthesis and Antibacterial Evaluation of an Indole Triazole Conjugate with In Silico Evidence of Allosteric Binding to Penicillin-Binding Protein 2a

doi: 10.3390/pharmaceutics17081013

Figure Lengend Snippet: Illustrates the time–kill curve of ITC against ( a ) MRSA (NCIM 5021), ( b ) MRSA (ATCC 43300).

Article Snippet: Notably, ITC displayed potent activity against MRSA strains (NCIM 5021: 0.156 ± 0.003 μg/mL) and (ATCC 43300: 0.115 ± 0.021 μg/mL), demonstrating enhanced efficacy against resistant strains compared to wild-type bacterial strains.

Techniques:

Journal: Pharmaceutics

Article Title: Synthesis and Antibacterial Evaluation of an Indole Triazole Conjugate with In Silico Evidence of Allosteric Binding to Penicillin-Binding Protein 2a

doi: 10.3390/pharmaceutics17081013

Figure Lengend Snippet: Illustrates the activity of ITC against MRSA (ATCC43300) pre-formed biofilm. p < 0.05 (*), p < 0.001 (***).

Article Snippet: Notably, ITC displayed potent activity against MRSA strains (NCIM 5021: 0.156 ± 0.003 μg/mL) and (ATCC 43300: 0.115 ± 0.021 μg/mL), demonstrating enhanced efficacy against resistant strains compared to wild-type bacterial strains.

Techniques: Activity Assay

Journal: bioRxiv

Article Title: Cancer cells transfer invasive properties through microRNAs contained in collagen-tracks

doi: 10.1101/2025.01.25.634857

Figure Lengend Snippet: (a) Immunofluorescence images of MDA-DDR1-GFP cells seeded on collagen III and non-fibrillar collagen I matrices by MDA-DDR1-GFP cells. Cells were imaged by confocal microscopy. DDR1 is stained in green, actin in red, nuclei in blue and collagen is imaged by internal reflection microscopy (IRM) in grey. Scale bar, 10 μm. (b) Immunofluorescence images of collagen-tracks formation by MDA-DDR1-GFP cells on type I collagen and treated or not with LOX-L2. DDR1 is stained in green, actin in red, nuclei in blue and collagen imaged by IRM in grey. Scale bar, 10 µm. (c) Quantification of collagen-tracks formation by MDA-DDR1-GFP cells on type I collagen and treated or not with LOX-L2. Values represent the mean +/- SEM of n=3 experiment (with 5 images per condition). (d) Immunofluorescence images of basal primary breast cancer cells or MCF-7 cells seeded on type I collagen during 24h and stained for DDR1 in green, actin in red, nuclei in blue and collagen is imaged by IRM in grey. Scale bar, 10 μm. (e) Immunofluorescence images of collagen-track formation by MDA-DDR1-GFP cells on type I collagen and treated or not with Mn2+. DDR1 is stained in green, actin in red, nucleus in blue and collagen imaged by IRM in grey. Scale bar, 10 µm. (f) Quantification of collagen-track formation by MDA-DDR1-GFP cells on type I collagen and treated or not with Mn2+. Values represent the mean +/- SEM of n=3 experiments (5 images per experiment and per condition).

Article Snippet: Type I collagen (rat tail, 354236) was purchased from Corning, type III collagen (5021) was purchased from CellSystems, fibronectin (F1056) was purchased from Sigma Aldrich and matrigel was purchased from Dutscher (#356231).

Techniques: Immunofluorescence, Confocal Microscopy, Staining, Microscopy